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. 2018 Oct 17;26(9):1383–1390. doi: 10.3727/096504018X15188340975709

Figure 5.

Figure 5

Knockdown of CCAL inhibited the activation of NOTCH1 pathway in PTC cells. Knockdown of CCAL significantly inhibited the (A) mRNA and (B) protein levels of NOTCH1 and the protein levels of its target genes (HEY1 and HEY2) in BCPAP and TPC-1 cells. (C) CCAL knockdown significantly inhibited the enrichment of histone active modification H3K4me3 on NOTCH1 promoter. ChIP assay was conducted with CCAL-silenced or control BCPAP cells. (D) RT-qPCR analysis indicated that NOTCH1 was restored in CCAL-depleted BCPAP and TPC-1 cells. (E) CCK-8 assays showed that overexpression of NOTCH1 rescued the proliferation of CCAL-depleted BCPAP and TPC-1 cells. Transwell assays indicated that overexpression of NOTCH1 rescued the (F) migration and (G) invasion of CCAL-depleted BCPAP and TPC-1 cells. *p < 0.05, **p < 0.01 and ***p < 0.001 by two-tailed Student’s t-test. All data presented are shown as means ± SD collected from three independent experiments.