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. 2018 Sep 14;26(8):1167–1174. doi: 10.3727/096504017X15051741798389

Figure 4.

Figure 4

Inositol polyphosphate 4-phosphatase B (INPP4B) is the target gene of miR-1290. (A) miR-1290 could bind to the 3′-untranslated region (3′-UTR) of INPP4B predicted using public software. The wild and mutated seed sites are shown. (B) Western blot assay revealed that miR-1290 had an opposite expression level in CRC cells. GAPDH was used as the loading control. (C) Luciferase reporter assay suggested that miR-1290 directly bound to the 3′-UTR of INPP4B, and the mutated seed sites abrogated the binding. (D, E) miR-1290 promoted cyclin D1 level and inhibited p27 level in CRC cells. Data are presented as mean ± SD for three independent experiments. *p < 0.05.