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. 2018 Mar 5;26(2):297–305. doi: 10.3727/096504017X14980882803151

Figure 2.

Figure 2

MEG3 overexpression suppresses proliferation and invasion of leukemia cells. (A) HL-60 cells were transfected with LV-GFP or LV-MEG3 for 24 h, and the mRNA levels of MEG3 were measured by qRT-PCR. (B) HL-60 cells were transfected with LV-GFP or LV-MEG3 for 24, 48, 72, and 96 h, and the cell viability was assayed by the CCK-8 kit. (C) Matrigel invasion assay. (D) Quantifications of (C). (E) Protein levels of proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (BCL-2), matrix metalloproteinase 9 (MMP9), and vascular endothelial growth factor (VEGF) were assayed by Western blot. (F, G) Quantifications of (E). All the experiments were repeated at least three times, and the representative data are shown. GAPDH was used as loading control. *p < 0.05, **p < 0.01 versus mock.