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. 2018 Oct 17;26(9):1391–1399. doi: 10.3727/096504018X15178798885361

Figure 3.

Figure 3

Annexin A2 receptor (ANXA2R) played a critical role in miR-139-3p modulating EMT process. (A) Diagram of the putative binding sequence of miR-139-3p in the 3′-untranslated region (3′-UTR) containing reporter constructs of ANXA2R is shown. (B) Luciferase reporter assays showed that miR-139-3p significantly decreased the luciferase activity of WT ANXA2R but not mutant (MUT) ANXA2R. **p < 0.05 compared with control. (C) Western blot assay showed that miR-139-3p downregulated ANXA2R expression in HepG2 cells, and anti-miR-139-3p upregulated the expression of ANXA2R in SNU-449 cells. (D) ANXA2R shRNA transfection in HepG2 cells eliminated the expression of E-cadherin in miR-139-3p overexpression cells. ANXA2R shRNA transfection in SNU-449 cells rescued the expression of E-cadherin in cells transfected with anti-miR-139-3p.