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Connection of miR-539 and TWIST1 on EMT regulation. (A, B) Protein levels of E-cadherin, α-SMA, and vimentin. Tubulin was used as a loading control. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001. pB-NC, empty pBABE-puro vector; pB-TWIST1, pB-NC carrying full-length wild-type TWIST1 gene.
