Figure 5.

Effect of MAGEA4 on EMT of esophageal cancer cells. (A) MAGEA4 protein expression after transfection with sh-NC or sh-MAGEA4. (B) Expression of E-cadherin, α-SMA, and vimentin after MAGEA4 knockdown. (C, D) Detection of E-cadherin, α-SMA, and vimentin expression in protein levels by Western blot assay. Tubulin was used as a loading control. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001. sh-MAGEA4, pSicoR vector carrying short hairpin RNAs targeting MAGEA4; pB-MAGEA4, pB-NC carrying full-length wild-type MAGEA4 gene.