Figure 3.

Baicalein suppresses the expression of cyclin D1 in HeLa cells and arrests cells at the G₀/G₁ phase. FACS was used to analyze the cell cycle in baicalein-treated HeLa cells, and a quantitative analysis of the cell cycle is shown. (A) The cell cycles of HeLa cells treated with baicalein at 0, 20, 40, and 80 μg/ml. (B) The quantitative analysis of (A). (C) The expression of cyclin D1 in HeLa cells treated with baicalein at 0, 20, 40, and 80 μg/ml, which was detected by Western blot. (D) The quantitative analysis of (C). The data are shown as the mean ± SD of three independent experiments (*p < 0.05, **p < 0.01 vs. control using one-way ANOVA).