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. 2018 Aug 23;26(7):1055–1062. doi: 10.3727/096504018X15152056890500

Figure 3.

Figure 3

miR-519d-3p regulates HIF-2α expression levels via targeting of its 3′-untranslated region (3′-UTR). (A) The putative binding site between HIF-2α 3′-UTR and miR-519d-3p was analyzed by TargetScan and miRanda. (B) Luciferase activity of wild-type (WT) or mutant (Mut) HIF-2α 3′-UTR in 293T cells transfected with miR-519d-3p mimic or NC (negative control). (C) HIF-2α mRNA in HeLa cells transfected with miR-519d-3p mimic or NC under hypoxic conditions was measured by RT-qPCR. (D) HIF-2α mRNA in HeLa cells transfected with miR-519d-3p mimic or NC under hypoxic conditions was measured by Western blotting. Data are presented as the mean ± standard deviation. ***p < 0.001 and ns versus NC.