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. 2018 Sep 14;26(8):1267–1274. doi: 10.3727/096504018X15172227703244

Figure 2.

Figure 2

B7-H4 has no significant impact on the growth of bladder cancer cells. (A) Left: Western blot analysis of B7-H4 protein levels in T24 and J82 cells transfected with the B7-H4-expressing plasmid or empty vector. Right: Western blot analysis of B7-H4 protein levels in T24 and J82 cells transfected with control small interfering RNA (C-siRNA) or B7-H4-targeting siRNA. (B) Cell proliferation determined by MTT assays in T24 and J82 cells transfected with the B7-H4-expressing plasmid or empty vector. (C) Colony formation assay. T24 and J82 cells stably transfected with the B7-H4-expressing plasmid or empty vector were cultured for 14 days to form colonies. (D) Cell proliferation determined by MTT assays in T24 and J82 cells transfected with C-siRNA or B7-H4-targeting siRNA. n.s., no significance.