Figure 4.
Overexpression of B7-H4 induces epithelial–mesenchymal transition (EMT) in bladder cancer cells. (A) Morphological changes in T24 cells with overexpression of B7-H4. Scale bar: 60 μm. (B) Western blot analysis of indicated proteins in T24 and J82 cells transfected with the B7-H4-expressing plasmid or empty vector. (C) Western blot analysis of indicated proteins in T24 and J82 cells transfected with C-siRNA or B7-H4-targeting siRNA. Numbers below Western blots represent fold change in protein levels after normalization to β-actin.