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miR-522-3p overexpression upregulated the expression of c-myc, cyclin E, and CDK2. miR-522-3p mimic, inhibitor, or negative control (mimic NC and inhibitor NC) was transfected into two human CRC cell lines (HCT-116 and HT29). (A) Protein expression of c-myc, cyclin E, and CDK2 in miR-transfected cells was assessed by Western blot analysis. Quantitative analysis of c-myc (B), cyclin E (C), and CDK2 (D) based on the results from Western blotting. *p < 0.05, **p < 0.01, ***p < 0.001.
