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. 2018 Aug 23;26(7):1113–1121. doi: 10.3727/096504018X15166199939341

Figure 4.

Figure 4

Bloom syndrome protein (BLM) was a target gene of miR-522-3p. miR-522-3p mimic, inhibitor, or negative control (mimic NC and inhibitor NC) was transfected into two human CRC cell lines (HCT-116 and HT29). Protein expression of BLM in HCT-116 cells (A) and HT29 cells (B) was assessed by Western blot analysis. (C) The predicted binding site in 3′-untranslated region (3′-UTR) of BLM, which can directly bind to miR-522-3p. (D) Luciferase reporter assay was performed to verify whether BLM was a target of miR-522-3p. ns, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.