Figure 5.

miR-522-3p inhibition reduced cell viability and promoted cell apoptosis in human CRC cells via upregulation of BLM. HCT-116 and HT29 cells were transfected with miR-522-3p inhibitor or si-BLM, or cotransfected with miR-522-3p inhibitor plus si-BLM. Cells cotransfected with inhibitor NC and si-NC acted as control. (A) Cell viability was detected by MTT assay. (B) Cell apoptosis was measured by flow cytometry detection. ns, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. si-BLM, BLM-specific targeted small interfering RNA; si-NC, nontargeted sequence.