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. 2018 Jun 11;26(5):713–723. doi: 10.3727/096504017X15016337254641

Figure 6.

Figure 6

Ephrin type B receptor 2 (EphB2) was a direct target of miR-204. (A) The potential binding site in 3′-UTR EphB2 of miR-204 was predicted by microRNA.org. (B) Relative luciferase activity was measured in Hela and C33A cell lines cotransfected with pGL3-EphB2-3′UTR WT or MU reporter plasmids and the miR-204 mimic or NC by luciferase report assay. (C) The protein expression levels of EphB2 in HeLa and C33A cell lines with the miR-204 mimic or NC transfection were determined by Western blot. *p < 0.05, **p < 0.01 versus the NC group.