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. 2018 Jul 5;26(6):827–835. doi: 10.3727/096504017X14934840662335

Figure 4.

Figure 4

miR-21 promoted the activation of CAFs by targeting PDCD4. PCDC4 and α-SMA expressions were measured in the murine CAFs transfected with different micro-RNAs and treated with TGF-β. (A, B) Sequences of miR-21 used to transfect human CAFs and murine CAFs, respectively. (C) PDCD4 expression was decreased in the CAFs with increased miR-21, but it was increased in the CAFs with miR-21 inhibitors. (D) In contrast to PDCD4, α-SMA expression was decreased in the murine CAFs with low miR-21 but was enhanced in the CAFs with high miR-21. (E) Murine CAFs transfected with PDCD4 siRNAs were treated with TGF-β to detect their activation by measuring α-SMA expression. The CAFs of the control groups were transfected with scrambled siRNA and treated with complete medium. The CAFs with PDCD4 deficiency had an increased α-SMA expression. (F) CAFs with the miR-21 inhibitor and PDCD4 deficiency showed an increased α-SMA compared with the CAFs with the miR-21 inhibitor alone. *p < 0.05; **p < 0.01; ***p < 0.001.