Cell division protein kinase 6 (CDK6) was a direct target of miR-4775. U-118 MG cells were transfected with miR-4775 mimic, miR-4775 inhibitor, mimic control, and inhibitor control. (A) The predicted CDK6 binding sites on miR-4775 were analyzed by TargetScan and the microRNA database. (B) Relative mRNA and protein levels of CDK6 were detected by qRT-PCR and Western blot. (C) The direct target of miR-4775 was examined by dual-luciferase activity assay. *p < 0.05, **p < 0.01, ***p < 0.001.