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. 2018 Jul 5;26(6):977–985. doi: 10.3727/096504017X15037506066252

Figure 2.

Figure 2

Bcl-2 was a direct target gene of miR-34a-5p in HeLa cells. (A) The predicted binding sequence between miR-34a-5p and the 3′-untranslated region (3′-UTR) of Bcl-2. (B) Cotransfection with pre-miR-34a-5p and Bcl-2-wt (wild type) decreased the relative luciferase activity, while the Bcl-2-mut (mutant) had no effect. (C) Suppression of miR-34a-5p promoted the mRNA expression of Bcl-2 in HeLa cells, while overexpression of miR-34a-5p inhibited the mRNA expression of Bcl-2. (D) Suppression of miR-34a-5p upregulated the protein levels of Bcl-2 in HeLa cells, while overexpression of miR-34a-5p inhibited the protein levels of Bcl-2. *p < 0.05.