Skip to main content
. 2018 May 7;26(4):605–616. doi: 10.3727/096504017X15009778205068

Figure 6.

Figure 6

Depletion of vimentin suppressed the migration and invasion capacities of KKU-214L5. KKU-214L5 cells were treated with short interfering RNA for vimentin (si-vimentin) or a scrambled sequence (sc), and the expression levels of vimentin were determined using (A) Western blotting 24–96 h posttreatment and (B) immunocytofluorescence stain 48 h posttreatment. Magnification: 200×; scale bar: 50 μm. (C) No significant differences in proliferation were found between si-vimentin-treated KKU-214L5 and the scramble control cells. (D, E) Treatment of si-vimentin significantly suppressed the migration and (F) invasion capacities of KKU-214L5 compared to the scramble control cells 48 h posttreatment. (G) Suppression of vimentin did not alter the expression levels of other EMT markers and the enzyme activities of MMPs. Protein lysates of 10 μg were used for all EMT markers, except ZO-1 and slug were at 30 μg. The numbers indicate the comparison of the band intensities by giving those of scramble control as 1. **p < 0.01. sc = scramble control cells; si = si-vimentin-treated cells.