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. 2018 Oct 17;26(9):1439–1446. doi: 10.3727/096504018X15189093975640

Figure 3.

Figure 3

High-mobility group box 1 (HMGB1) is a direct target gene of miR-935 in OS. (A) Sequence alignment of wild-type (WT) and mutant (MUT) putative miR-935 binding sites in the 3′-untranslated region (3′-UTR) of HMGB1. (B) Luciferase activity was detected in MG-63 and Saos-2 cells cotransfected with miR-935 mimic or miR-NC and pMIR-HMGB1-3′-UTR WT and pMIR-HMGB1-3′-UTR MUT. Relative mRNA (C) and protein (D) levels of HMGB1 in MG-63 and Saos-2 cells after transfection with miR-935 mimics or miR-NC were determined using RT-qPCR and Western blot analysis, respectively. *p < 0.05.