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. 2018 Oct 17;26(9):1439–1446. doi: 10.3727/096504018X15189093975640

Figure 5.

Figure 5

Recovery of expression of HMGB1 rescues the suppressive effects of miR-935 overexpression in OS cell proliferation and invasion. MG-63 and Saos-2 cells were cotransfected with miR-935 mimic and pCMV-HMGB1 or pCMV and were used in the following experiments. (A) Relative protein expression of HMGB1 in indicated cells was determined using Western blot analysis. Cell proliferative (B) and invasion (C) abilities in the above-mentioned cells were evaluated using MTT and Transwell invasion assays, respectively. *p < 0.05. #p < 0.05.