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. 2018 Oct 17;26(9):1327–1334. doi: 10.3727/096504018X15165493852990

Figure 3.

Figure 3

IRF2 and CBX7 were the direct targets of miR-18a in HCC. (A) The predicted miR-18a binding sequence in the IRF2 3′-untranslated region (3′-UTR) and the mutated sequence. (B) The predicted miR-18a binding sequence in CBX7 3′-UTR and the mutated sequence. (C) Relative luciferase activity in the Bel-7402 cell line transfected with wild-type IRF2 3′-UTR (WT) or mutated IRF2 3′-UTR (Mut). (D) Relative luciferase activity in the Bel-7402 cell line transfected with CBX73′-UTR WT or CBX73′-UTR Mut. (E) Expression of IRF2 in the Bel-7402 cell line transfected with miR-18a mimic or NC miRNA was examined by Western blot assay. (F) Expression of CBX7 in Bel-7402 cell line transfected with miR-18a mimic or NC miRNA was examined by Western blot assay. **p < 0.01. NC miRNA, negative control microRNA; IRF2, interferon regulatory factor 2; CBX7, chromobox protein homolog 7.