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. 2018 Aug 23;26(7):1093–1102. doi: 10.3727/096504018X15154112497142

Figure 3.

Figure 3

Mitogen-activated protein kinase 1 (MAPK1) is a direct target of miR-212 in PCa. (A) Predicted miR-212 target sequences in the 3′-untranslated regions (3′-UTRs) of MAPK1 and positions of mutated nucleotides (bold) in the 3′-UTR of MAPK1. (B, C) Relative luciferase activities in DU145 and PC3 cells cotransfected with miR-212 mimics or miR-NC and pmirGLO-MAPK1-3′-UTR wild type (Wt) (1 and 2) or pmirGLO-MAPK1-3′-UTR mutant (Mut) (1 and 2) were detected using a Dual-Luciferase Reporter assay system. Changes in MAPK1 mRNA (C) and protein (D) expression in DU145 and PC3 cells after transfection with miR-212 mimics or miR-NC were determined with qRT-PCR and Western blot analysis respectively. *p < 0.05 versus their respective control.