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. 2018 Mar 5;26(2):249–259. doi: 10.3727/096504017X14953948675430

Figure 6.

Figure 6

miR-186 inhibited the NF-κB signaling pathway via downregulating SENP1 in RCC cells. (A) The expression of key components of the NF-κB signaling pathway and its downstream factors in ACHN cells cotransfected with the SENP1 vector and miR-186 mimic was measured by Western blot analysis. Control group: miR-NC + vector; miR-186 group: vector + miR-186; SENP1 group: miR-NC + SENP1; miR-186 + SENP1 group: miR-186 + SENP1. GAPDH was used as internal control. (B) The inhibitory effect of pyrrolidine dithiocarbamate (PDTC) on ACHN cell viability was determined by MTT assay. ACHN cells were treated with different concentrations of PDTC for 24 h. (C) Invasive ability of ACHN cells pretransfected with the SENP1 vector or miR-186 mimic and treated with or without 50 nmol/L PDTC for 24 h was measured by Transwell assay. *p < 0.05 versus the control group.