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. 2018 May 7;26(4):625–635. doi: 10.3727/096504017X14953948675395

Figure 3.

Figure 3

Effect of lncCAMTA1 on MDA-MB-231 cells via negative regulation of miR-20b expression. Human breast cancer cell line MDA-MB-231 cells were transfected with lncCAMTA1 shRNA (sh-CAMTA1) and/or miR-20b inhibitor. (A) Expression levels of miR-20b were measured by qPCR after miR-20b mimic or miR-20b inhibitor transfection in MDA-MB-231 cells. (B) Trypan blue dye exclusion results for the relative viability of cells after corresponding treatment. (C) Relative number of migrated cells based on the Transwell assay. (D) Relative number of invasive cells based on the invasion assay. (E) Relative number of apoptotic cells measured by flow cytometry. (F) Protein expression levels of apoptotic-related factors measured by Western blotting. GAPDH acted as internal control. *p < 0.05, **p < 0.01, ***p < 0.001.