Long noncoding RNA activated by transforming growth factor-β (lncRNA-ATB) promoted cell proliferation, migration, and invasion in T24 cells. Human bladder cancer T24 cells were first transfected with pEX-lncRNA-ATB and sh-lncRNA-ATB. (A) Expression levels of lncRNA-ATB stably overexpressed or suppressed in T24 cells were detected by quantitative real-time (qRT)-PCR. (B) Cell viability, (C) migration, (D) invasion, (E) apoptosis, and (F) apoptosis-associated factors were examined by trypan blue exclusion, Transwell assay, flow cytometry, and Western blot, respectively. *p < 0.05, **p < 0.01, ***p < 0.001.