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. 2018 Jul 5;26(6):837–846. doi: 10.3727/096504017X14920318811721

Figure 1.

Figure 1

Long noncoding RNA X-inactive specific transcript (lncRNA XIST) is upregulated in osteosarcoma (OS) tissues and cell lines and promotes the proliferation and invasion of OS cells. (A, B) XIST expression was significantly increased in OS tissues when compared with the normal counterparts using the Disease-Related Human LncRNA Profiler and quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), respectively. (C) The four OS cell lines U2OS, Saos-2, MG63, and MNNG/HOS all had a higher level of XIST expression than the normal osteoblast cell line HFOB 1.19. (D) XIST knockdown was achieved by XIST small interfering RNA (si-XIST), and the inhibitory efficiency was verified by real-time PCR. (E, F) MTT assays revealed that knockdown of XIST attenuated the growth of both Saos-2 and MG63 cell lines for up to 3 days, compared with the si-NC (negative control) group. (G) Cell invasiveness in Saos-2 and MG63 cells transfected with si-XIST or si-NC was detected by Transwell invasion assay and is shown both pictorially and graphically. *p < 0.05, **p < 0.01 compared to normal tissue in (B), HFOB 1.19 cells in (C), and the si-NC groups in (D–G).