Skip to main content
. 2018 Jul 5;26(6):837–846. doi: 10.3727/096504017X14920318811721

Figure 2.

Figure 2

XIST is associated with microRNA-320b (miR-320b) expression in OS cells. (A) Real-time PCR assay showed that knockdown of XIST (si-XIST) caused upregulation of miR-320b in the Saos-2 and MG63 cell lines. (B) XIST expression was decreased in response to miR-320b overexpression, compared with the miR-NC (NC) group. Data are presented as mean ± SD of three independent experiments. (C) Generation of XIST-WT and XIST-MUT containing luciferase reporter vectors by sequentially mutating the predicted miR-320b binding site in the XIST 3′-untranslated region (3′-UTR). (D) The XIST-WT/XIST-MUT vectors and miR-NC/miR-320b mimics were cotransfected into human embryonic kidney (HEK) 293T cells, respectively. Luciferase activity of the XIST-WT vector was reduced in cells cotransfected with miR-320b mimics. Repression of luciferase activity by miR-320b was not shown in cells transfected with XIST-MUT. Data are presented as mean ± SD of three independent experiments. (E) An inverse correlation between XIST and miR-320b expression was observed. *p < 0.05 compared to the NC group.