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. 2018 Oct 17;26(9):1375–1382. doi: 10.3727/096504018X15188352857437

Figure 2.

Figure 2

miR-365 overexpression suppressed CCA cell proliferation but induced cell apoptosis. (A) CCALP1 and SG231 cells were transfected with miR-365 mimic to overexpress miR-365. Cell proliferation was determined in CCALP1 and SG231 cells transfected with miR-365 mimic or controls by (B) cell counting kit-8 (CCK-8) assay and (C) colony formation assay. (D) Percentages of cells in the S phase were determined by FACS. (E) Cell apoptosis was measured in CCALP1 and SG231 cells transfected with miR-365 mimic or controls by staining with annexin V/propidium iodide (PI). Data are presented as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.