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. 2018 Oct 17;26(9):1375–1382. doi: 10.3727/096504018X15188352857437

Figure 4.

Figure 4

E2F2 was a target of miR-365. (A) The miR-365 binding site in the 3′-untranslated region (3′-UTR) region of E2F2 mRNA was predicted by bioinformatics analysis. (B) Luciferase reporter assays were performed using CCALP1 and SG231 cells cotransfected with the miR-365 mimic or control and E2F2-wt or E2F2-mut 3′-UTR reporter plasmid. Analysis of (C) E2F2 mRNA and (D) protein levels in CCALP1 and SG231 cells transfected with miR-365 mimic or controls by RT-qPCR. (E) Spearman’s correlation analysis was used to determine the correlations between the levels of miR-365 and E2F2 in human CCA tissues (n = 49). Data are presented as means ± SD. **p < 0.01 and ***p < 0.001.