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. 2018 Aug 23;26(7):1015–1022. doi: 10.3727/096504018X15151486241153

Figure 3.

Figure 3

Lactate dehydrogenase A (LDHA) was a direct target of miR-200c. (A) The putative binding sequence for miR-200c in the 3′-untranslated region (3′-UTR) of LDHA. The luciferase activity assay revealed the miR-200c mimic suppressed LDHA 3′-UTR wild-type (WT) luciferase activity, whereas it had no effect on LDHA 3′-UTR mutant (Mut) luciferase activity compared with control in (B) A549 and (C) NCI-H460 cells. ***p < 0.001. Protein expression of LDHA in (D) A549 and (E) NCI-H460 cell line after miR-200c mimic and NC miRNA transfection was detected by Western blot assay. (F) The expression of LDHA was inversely correlated with the expression of miR-200c in NSCLC.