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. 2018 Oct 17;26(9):1317–1326. doi: 10.3727/096504018X15179680859017

Figure 3.

Figure 3

Notch1 is a direct target of miR-708 in gastric cancer. (A) The putative wild-type (Wt) and mutant (Mut) miR-708 binding sequences in the 3′-untranslated region (3′-UTR) of Notch1 are shown. (B, C) BGC-823 and SGC-7901 cells were cotransfected with miR-708 mimics or miR-NC and luciferase reporter plasmid carrying Wt or Mut binding sites for miR-708 in the 3′-UTR of Notch1. Then 48 h posttransfection, cells were harvested and subjected to the analysis of luciferase activity using dual-luciferase reporter assays. *p < 0.05 compared with miR-NC. (D, E) RT-qPCR and Western blot analysis were conducted to measure the expression of Notch1 at the mRNA and protein levels, respectively, in BGC-823 and SGC-7901 cells transfected with miR-708 mimics or miR-NC. *p < 0.05 compared with miR-NC.