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. 2021 Jan 29;19:33. doi: 10.1186/s12951-021-00768-w

Table 1.

Primer sequences of amplification VHH genes for recombinant plasmids pcDNA3.1-Nbs-hFc construction

Primer Sequences
Nbs-F 5′-CAGGGATCCCAGGTGCAGCTGGTGGAGTC-3′
Nbs-R 5′-CGCCTCGAGTGAGGAGACGGTGACCTGGG-3′
biNbs-R1 5′-TGAACCGCCTCCACCGCTGCCGCCTCCGCCTGAGGAGACGGTGACCTGGG-3′
biNbs-F2 5′-GGTGGAGGCGGTTCAGGAGGTGGCGGATCTCAGGTGCAGCTGGTGGAGTC-3′
triNbs-R32 5′-AGATCCGCCACCTCCGCTGCCGCCTCCGCCTGAGGAGACGGTGACCTGGG-3′
triNbs-F3 5′-GGAGGTGGCGGATCTGGTGGAGGCGGTTCACAGGTGCAGCTGGTGGAGTC-3′

Underline indicates position of restriction enzyme sites