Figure 1.

Effects of glycosyltransferases on S100A8/A9-induced migration and invasion. Migration (left) and invasion (right) of WM-115 cells transfected with indicated genes [green fluorescence protein (GFP) as a control] were assessed by the Boyden chamber method. The transfected WM-115 cells were placed in the insert chamber, and purified recombinant S100A8/A9 (final concentration of 100 ng/ml) was added to the bottom well. The quantified results were viewed from the upper surface of the insert chamber membrane, and representative images of migrating or invading cells were obtained using crystal violet staining at the lower side. Data are shown as means ± SD. *p < 0.05 and **p < 0.01.