Figure 2.

Effects of GCNT3 (β-1,3-galactosyl-O-glycosyl-glycoprotein β-1,6-N-acetylglucosaminyltransferase 3) combined with each S100 soil sensor receptors (SSSR) on S100A8/A9-induced migration and invasion. Migration (A) and invasion (B) of WM-115 cells transfected with indicated combinations of the genes [empty vector (EV) and GFP used as controls] were assessed by the Boyden chamber method. The transfected WM-115 cells in the top chamber were stimulated or not stimulated with the purified recombinant S100A8/A9 (final concentration of 100 ng/ml). Data are shown as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001.