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. 2018 Apr 10;26(3):431–444. doi: 10.3727/096504017X15031557924123

Figure 4.

Figure 4

Effect of inhibition of intrinsic GCNT3 on S100A8/A9-induced migration and invasion. (A) Protein levels of MCAM and GCNT3 were compared between WM-115 and WM-266-4 cells by Western blotting. Protein levels of MCAM were determine by Western blotting after treatment of WM-266-4 cells with the indicated siRNAs (final concentration of 20 nM) (B) and with talniflumate (Tal) (final concentration of 100 μM) (C). After treatment of WM-266-4 cells with the indicated siRNAs (final concentration of 20 nM) (D) or Tal (final concentration of 100 μM) (E), migration of the treated cells was assessed by the Boyden chamber method. The treated cells in the top chamber were stimulated or not stimulated with purified recombinant S100A8/A9 (final concentration of 100 ng/ml). The quantified results are displayed on the left, and representative images of migrating cells detected by crystal violet staining are shown on the right. Data are shown as means ± SD. *p < 0.05, ***p < 0.001.