Figure 4.

miR-206 reduces CC cell migration and invasion. (a) Wound healing assay performed with SiHa and HeLa cells over 48 h. Cells and wounds were pretreated as described above. Wound healing within the scrape line was recorded every day. Representative scrape lines are shown at day 3; dashed line indicates the margin of the scratch at day 1. (b) Representative fields (magnification: 10×) showing invasive cells after 24-h culture in Matrigel invasion chambers. Panels show SiHa and HeLa cell invasion after transfection with miR-206 mimics or miR-NC. Quantitative analysis of cell invasion experiments demonstrates that miR-206 decreases SiHa and HeLa cell invasion compared to miR-NC. (c) Relative MMP2/9 expressions in SiHa and HeLa cells were measured after the cells were transfected with miR-206 mimics or miR-NC using Western blot. Results shown are the mean ± SD of three repeated independent experiments. *p < 0.01, compared with miR-NC, Student’s t-test.