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. 2018 Apr 10;26(3):363–372. doi: 10.3727/096504017X14953948675421

Figure 4.

Figure 4

(A) The gene sequences of suppressor of TUSC5 regulated by miR-3188. (B) The relative luciferase activities in the wild-type 3′-UTR of TUSC5 and mutant-type 3′-UTR of TUSC5 in transfected cells. (C) The relative expression level of TUSC5 in transfected cells detected by qRT-PCR and Western blot. (D) The relative expression level of TUSC5 after cells were transfected with si-TUSC5. (E) Apoptosis assay after cells were transfected with si-TUSC5. (F) The relative expression levels of apoptosis-related proteins (Bcl-2, Bax, and caspase 3) after cells were transfected with si-TUSC5. (G) Cell migration assay after cells were transfected with si-TUSC5. (H) The relative expression levels of N-cadherin, E-cadherin, vimentin, and Snail after cells were transfected with si-TUSC5. *p < 0.05, **p < 0.01 compared with control; #p < 0.05 compared with the miR-3188 inhibitor group.