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. 2018 Jan 19;26(1):131–143. doi: 10.3727/096504017X14944585873631

Figure 5.

Figure 5

The interaction between SChLAP1 and miR-198 influences the progression of prostate cancer. (A, B) LNCap and PC-3 cells were transfected with SChLAP1-siRNA for 24 h, and the levels of miR-198 were measured by Northern blot (A) and qRT-PCR (B). (C) PC-3 cells were transfected with SChLAP1-siRNA and/or the miR-198 inhibitor for 24 h, and the levels of miR-198 were measured by Northern blot. (D) PC-3 cells were transfected with synthetic SChLAP1 and/or the miR-198 mimic for 24 h, and the levels of miR-198 were measured by Northern blot. (E) PC-3 cells were transfected with wild-type or mutant SChLAP1 reporter plasmid and cotransfected with the miR-198 mimic for 24 h. Cell lysates were assayed for luciferase activity. (F) Quantification of cell viability assay. (G) Quantification of cell apoptosis assay. (H) Quantification of cell migration. (I) Quantification of cell invasion. All experiments were repeated at least three times. GAPDH was used as a loading control. **p < 0.01, ***p < 0.001.