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. 2021 Jan 28;22:90. doi: 10.1186/s12864-021-07396-6

Fig. 8.

Fig. 8

Expression analysis of osmotic and ABA-related genes in VC and RcDREB2B overexpressed lines. Analysis of the osmotic stress-responsive genes and ABA-related genes in VC and RcDREB2B transgenic lines was performed using RT-qPCR. Total RNA extraction was carried out from 3-week-old seedlings grown under normal growth conditions. Three separate replicates were carried out for RT-qPCR analysis. The relative expression of individual genes of VC was set to 1.0, and AtACTIN2 was used as an internal control gene. An additional file (Additional file 2: Table S2) lists the primers used. Different letters indicate significant differences in three independent experiments at P < 0.05 using one-way ANOVA analysis