Table 1.
Samples profile and phenotypic and molecular features of actinomycetes hospital environmental isolates
| Isolates | Sample profile | Phenotypic features | 16S rRNA analysis | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of isolates | Designation | Sample type | Hospital department | Tm | TDS for water samples | pH | Opt. Tm | Growth rate | Pigment production | Nitrate reduction | Catalase | Tween 80 hydrolyses | Urease | Lysozyme Resistance | Decomposition of Tyrosine | Decomposition of Xanthene | Decomposition of Hypoxanthine | Similarity (%) | Base pair differences | Identification |
| 5 | AE13/AE14/AE15/AE16/AE17 | Soil/Drinking water | Men Surgery / Emergency | 7–14 | 200 | 7.4 | 35 | R | N | + | + | – | + | – | – | – | – | 100 | 0/925 | M. setense |
| 2 | AE20/AE21 | Soil/Well water | Pediatrics / Maternity | 12–21 | 582 | 7.6 | 35 | S | N | – | – | – | – | – | – | – | – | 100 | 0/830 | M. lentiflavum |
| 1 | AE4 | Shower water | Women Surgery | 20 | 312 | 7.2–7.8 | 35 | S | N | – | + | – | + | – | – | – | – | 99.9 | 1/640 | M. conceptionense |
| 2 | AE2/ AE3 | Dust /Soil | ICU/ Oncology | 20–26 | 8 | 35 | R | White | + | – | + | – | – | – | – | – | 100 | 0/735 | M. mucogenicum | |
| 1 | AE26 | Drinking water | General | 18 | 192 | 7.4 | 30 | R | White | + | – | + | – | – | – | – | – | 100 | 0/870 | M. septicum |
| 4 | AE5/AE6/AE7/AE8 | Dust/Soil/ Drinking Water | Men Surgery/ Maternity | 14–21 | 265 | 7–8.2 | 35 | R | N | + | – | – | – | – | – | – | – | 100 | 0/959 | M. fortuitum |
| 2 | AE18/ AE19 | Soil | Emergency/ ICU | 22–26 | 6.9 | 35 | S | N | + | – | + | – | – | – | – | – | 100 | 0/959 | M. chelonae. | |
| 3 | AE23/AE24/AE25 | Drinking Water/ Shower water | Neurosurgery/ Ophthalmology | 20–30 | 168–245 | 7.2–8 | 35 | S | Orange | + | + | – | – | – | – | – | – | 100 | 0/860 | M. gordonae |
| 1 | AE42 | Soil | Women Surgery | 33 | 7.6 | 35 | S | Pinkish | + | – | – | – | + | – | – | – | 100 | 0/790 | N. rhamnosophilia | |
| 1 | AE39 | Soil | Pediatrics | 30 | 7.6 | 30 | R | – | + | – | – | + | – | – | – | 100 | 0/830 | N. bravicatena | ||
| 1 | AE9 | Dust | Emergency | 9 | 7.3 | 30 | R | Yellow | + | + | – | + | – | – | – | – | 100 | 0/830 | M. flavescens | |
| 2 | AE11/AE12 | Soil/Drinking water | Ophthalmology | 8–32 | 96 | 6.7–8 | 35 | R | N | + | + | – | + | – | – | – | – | 99.9 | 1/977 | M. massiliense/abscessus complex |
| 1 | AE22 | Well water | Green space | 13 | 412 | 7 | 35 | R | N | – | + | – | – | – | – | – | – | 99.9 | 1/880 | M. arupense |
| 2 | AE40/AE41 | Soil/Shower water | Emergency/ Oncology | 12–22 | 212 | 8.2 | 35 | R | Pink | – | – | + | + | + | – | – | – | 100 | 0/847 | N. asteroides |
| 2 | AE34/AE35 | Dust | Men Surgery/ICU | 8–21 | 7.8 | 35 | R | Orange | – | + | – | + | + | – | – | – | 99.78 | 2/800 | N. farcinica | |
| 1 | AE10 | Water | Infectious diseases | 25 | 8 | 30 | S | – | + | – | – | – | – | – | 100 | 0/920 | M. doricum | |||
| 3 | AE36/AE37/AE38 | Soil/Drinking water | Men surgery/ Infectious disease | 8–16 | 156 | 6.6–7.4 | 35 | R | White | + | + | – | – | + | – | – | – | 100 | 0/912 | N. cyriacigeorgica |
| 1 | AE1 | Soil | Emergency | 13 | 8.2 | 35 | R | Yellow | – | + | + | – | – | – | – | – | 99.9 | 1/932 | M. frederiksbergense | |
| 1 | AE43 | Soil | Pediatrics | 13 | 7.1 | 25 | R | Yellow | + | + | – | + | – | + | + | + | 100 | 0/694 | S. heliomycini | |
| 1 | AE44 | Soil | Emergency | 16 | 8 | 30 | R | Red | – | + | – | + | – | – | – | + | 100 | 0/763 | S. albus | |
| 1 | AE45 | Dust | Surgery | 21 | 7.8 | 30 | R | White | + | + | – | + | – | + | + | – | 100 | 0/940 | S. albogriseolus | |
| 4 | AE27/AE28/AE29/AE30 | Soil/Drinking water | Men Surgery / Emergency | 14–22 | 112 | 6.3 | 30 | R | White | + | + | + | + | – | + | + | – | 100 | 0/853 | R. erythropolis |
| 2 | AE31/AE32 | Soil/Shower water | Pediatrics / Maternity | 28 | 195 | 6.8 | 30 | R | Yellow | + | + | + | – | + | + | + | 99.9 | 2/993 | R. equi | |
| 1 | AE33 | Well Water | Women Surgery | 21 | 523 | 7.2 | 30 | R | N | – | + | – | + | – | – | – | + | 100 | 0/685 | R. facians |
| 1 | AE46 | Soil | Infectious diseases | 13 | 7.3 | 30 | R | White | – | + | – | + | – | – | + | – | 100 | 0/745 | A. globiformis | |
| 2 | AE47/AE48 | Drinking Water | Men surgery/ Infectious disease | 16 | 143 | 6.7–8 | 30 | R | Reddish | + | + | – | – | – | – | – | – | 100 | 0/960 | L. shinushuensis |
| 1 | AE48 | Dust | Emergency | 21 | 7 | 30 | R | White | + | – | + | – | – | – | – | – | 100 | 0/800 | G. terae | |
| 1 | AE49 | Soil | Pediatrics | 14 | 8.2 | 30 | R | White | + | + | – | – | + | – | – | – | 100 | 0/850 | D. maris | |
1; Tm; Temperature
2; R; Rapid grower < 7 days; S: Slow grower > 7 days
3; Similarity; % similarity to the nearest validated species
4; Base pair differences: the number of nucleotide differences between isolates and the nearest validated species
5. TDS: total dissolved solids