Fig. 2.

Indel sizes and frequencies elicited by TRAC and CD52 gRNAs in 293 T cells, as analyzed by TIDE and IDAA. The TRAC and CD52 gRNA/Cas9-expressing cell populations FACS-isolated from P4 of Fig. 1 were analyzed by TIDE and IDAA for indels elicited at the respective gRNA target sites. a) TIDE shows indels as bars in a graph, indicating indel size in bp on the x-axis and indel frequency on the y-axis. Wild-type allele is indicated by pink color, whereas indels are shown in red (p< 0.001) or black (p> 0.001), indicating significance of detection. b) IDAA shows amplicons derived from the gRNA target sites as peaks, indicating amplicon size in bp, from which indel size can be deduced, on the x-axis and amplicon amount (= indel frequency) on the y-axis. Wild-type allele is indicated by orange color, whereas frame-shifting indels of > 5% frequency are shown in blue and the remainder in white color