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. 2021 Jan 29;21:9. doi: 10.1186/s12896-020-00665-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Transfection efficiency of pSpCas9-2A-GFP plasmid in human primary T cells. Three days after electroporation of activated human primary T cells with no plasmid or pSpCas9-2A-GFP/TRAC gRNA plasmid, the cells were analyzed by flow cytometry in the GFP channel. Analysis of the mock transfected cells was used to estimate the autofluorescence level of the cells in the GFP channel (marked by a bar in the graphs). The fraction of the mock transfected cells above this level (~ 2%) was subtracted from the fraction of the pSpCas9-2A-GFP transfected cells above this level (~ 26%), yielding an estimated transfection efficiency of ~ 24%