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. 2021 Jan 29;21:9. doi: 10.1186/s12896-020-00665-4

Table 1.

Oligos used for introduction of gRNA sequences for TRAC and CD52 genes into pSpCas9-2A-GFP plasmid

Name Sequence

TRAC-gRNA (F/top)

TRAC-gRNA (R/bottom)

5′p-CACCGTCTCTCAGCTGGTACACGGC-3′

5′p-AAACGCCGTGTACCAGCTGAGAGAC-3′

CD52-gRNA (F/top)

CD52-gRNA (R/bottom)

5′p-CACCGCAGCCTCCTGGTTATGGTAC-3′

5′p-AAACGTACCATAACCAGGAGGCTGC-3′

The gRNA sequences are underlined