Figure 1.

Gene expression of NETosis regulators PADI4, ELANE, and MPO in granulocytes from patients with SCD and healthy individuals. Results are presented as median relative quantifications of gene expression normalized by ACTB and GAPDH as reference genes. Both PADI4 (A) and MPO (C) mRNA expression increased only during acute VOC when compared with healthy volunteers. In contrast ELANE (B) mRNA expression increased in both VOC and steady state when compared with healthy volunteers. Kruskal‐Wallis test was performed with Dunn postcomparison correction. The numbers of patients studied are indicated in each panel. (D) Regression coefficients from multivariable linear regression analysis of the association of patient group with gene expression of each NETosis regulator, adjusted for white blood cell count. CI, confidence interval; ELANE, neutrophil elastase; MPO, myeloperoxidase; PADI4, peptidyl arginine deaminase 4; SCD, sickle cell disease; VOC, vaso‐occlusive crisis