Table 2.
Comparison of analytic performances of in‐house FRETS‐VWF73 and HemosIL AcuStar ADAMTS13 Activity assays
| FRETS‐VWF73 assay | HemosIL AcuStar ADAMTS13 Activity assay | |
|---|---|---|
| Level of automation | Highly manual | Fully automated |
| Substrate | FRETS‐VWF73 | GST‐VWF73 |
| Detection principle | Fluorescence | Chemiluminescence |
| Limit of detection | 10 IU/dL | 0.2 IU/dL |
| Contamination | Not applicable | None |
| Intra‐assay precision (CV) | 6.9% | 5.6% |
| Interassay precision (CV) | 10.2% | 9.7% |
| Assay length a | 1 hour | 33 minutes b |
Abbreviations: CV, coefficient of variation.
a
This duration does not include sample preparation, calibration and data analysis.
b
This is the time that the HemosIL AcuStar ADAMTS13 activity assay needs to assess the first sample; each subsequent sample will take an extra minute.