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. 2020 Dec 18;11:568006. doi: 10.3389/fphar.2020.568006

TABLE 1.

Primers used for qRT-PCR.

Genes Forward Reverse
GAPDH GGC​AAA​TTC​AAC​GGC​ACA​GT AGA​TGG​TGA​TGG​GCT​TCC​C
ACC GCC​TCC​GTC​AGC​TCA​GAT​AC ATG​TGA​AAG​GCC​AAA​CCA​TC
FABP4 CTT​TGC​CAC​AAG​GAA​AGT​GG TCC​CCA​TTT​ACG​CTG​ATG​AT
FATP4 ACT​GTT​CTC​CAA​GCT​AGT​GCT GAT​GAA​GAC​CCG​GAT​GAA​ACG
SREBP-1c GGA​GCC​ATG​GAT​TGC​ACA​TT GGCCCGGGAAGTCACTGT
PPaRγ CTGACAGGACTGTGTGAC TCT​GTG​TCA​ACC​ATG​GTA​AT
PPaRα TGC​AAA​CTT​GGA​CTT​GAA​CG AGG​AGG​ACA​GCA​TCG​TGA​AG
CYP4A10 AGG​TGT​GGC​CAA​ATC​CAG​AG AAT​GCA​GTT​CCT​GGC​TCC​TC
CYP4A14 ACC​CTC​CAG​CAT​TTC​CCA​TG CTG​TAA​GCA​GGC​ACT​TGG​GA
ACOX1 CTG​GTG​GGT​GGT​ATG​GTG​TC AAT​CTG​GCT​GCA​CGT​AGC​TT
CPT1α GTG​AAA​AGC​ACC​AGC​ACC​TG GAA​AGG​TGA​GTC​GAC​TGC​CA
PPARβ/δ CCT​CCA​TCG​TCA​ACA​AAG​ACG TTT​AGC​CAC​TGC​ATC​ATC​TGG​GCA​TGC​TC
METTL3 AGC​AGA​GCA​AGA​GAC​GAA​TTA​TC GGT​GGA​AAG​AGT​CGA​TCA​GCA
METTL14 AGA​GAA​ACC​TGC​AGG​GCT​TC TCC​TCC​TGC​TGC​ATT​TCC​AG
FTO TTC​ATG​CTG​GAT​GAC​CTC​AAT​G GCC​AAC​TGA​CAG​CGT​TCT​AAG
ALKBH5 CGC​GGT​CAT​CAA​CGA​CTA​CC ATG​GGC​TTG​AAC​TGG​AAC​TTG
YTHDF1 ATG​CCC​AAC​CTA​CTT​CTG​CC GAA​CAC​CCG​CCC​ACT​CTT​AA
YTHDF2 GAG​CAG​AGA​CCA​AAA​GGT​CAA​G CTG​TGG​GCT​CAA​GTA​AGG​TTC
YTHDF3 ATG​CCC​AAC​CTA​CTT​CTG​CC GAA​CAC​CCG​CCC​ACT​CTT​AA

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACC, acyl-CoA carboxylase; FABP4, fatty acid-binding protein 4; FATP4, fatty acid transporter protein 4; SREBP-1c, sterol regulatory element binding protein-1c; PPARα, peroxisome proliferator-activated receptor alpha; PPARγ, peroxisome proliferator-activated receptor gamma; CYP4A10, cytochrome P450, family 4, subfamily a, polypeptide 10; CYP4A14, cytochrome P450, family 4, subfamily a, polypeptide 14; ACOX1, acyl-CoA oxidase 1; CPT1α, carnitine palmitoyltransferase 1 alpha; PPARβ/δ, peroxisome proliferator-activated receptor beta/delta; METTL3, methyltransferase like 3; METTL14, methyltransferase like 14; FTO, fat mass and obesity associated; ALKBH5, alkB homolog 5; YTHDF1, YTH domain family 1; YTHDF2, YTH domain family 2; YTHDF3, YTH domain family.