Fig. 3.

Inhibition of MALAT1 level could protect brain injury and improve neuronal function in CIRI-treated mice. a MALAT1 expression under CIRI condition for 24 and 48 h was determined by RT-qPCR. b The role of interference of MALAT1 on the neuronal impairment was assessed by neurological deficit scores. c Western blot determined the expression of AQP4 was downregulated after transfection with MALAT1 siRNA under CIRI condition. d The effect of MALAT1 siRNA on the focal ischemia of brain sections and quantitative analysis of brain infarct volumes were shown by TTC staining in the CIRI model. CIRI, cerebral ischemia reperfusion injury; TTC, 2,3,5-triphenyltetrazolium chloride.