Points addressed in Annex II	Element	Description of Element	Information provided in surveillance report	Requirement fulfilled	Comments
Type and sensitivity of the detection method	Type of test	The diagnostic test used for the detection of EM must be defined. Modifications of the original method should be indicated	Yes	Yes	NA
	Test sensitivity	The sensitivity and specificity of the test used in the surveillance system must be reported. This would ideally be estimates from each participating laboratory reported as a point estimate (average) of the values across the country with minimum and maximum values or a probability distribution. Alternatively, a value of 0.78, as recommended by EFSA (2015), shall be used	Yes	Yes	NA
Selection of the target population	Definition of susceptible host population targeted by the system	The susceptible wild definitive host population(s) targeted by the surveillance system should be described and the choice justified. If domestic host species are sampled, evidence for the absence of wild definitive hosts and for these domestic animals having had access to outdoors should be provided	Yes	Yes	NA
	Size of susceptible host population targeted by the system	The size of the targeted (wildlife) population should be reported, together with the evidence for this. Historical population data should be updated since these may not reflect current populations	Yes	Yes	NA
Sampling strategy	Epidemiological unit	It should be clearly defined if individual animals or individual faeces samples collected from the environment constitute the epidemiological unit. If individual faeces samples are collected from the environment, the method applied to establish the species from which the faeces originated has to be reported	Yes	Yes	NA
	Sample size calculation	The applied survey design should be fully documented, including considerations regarding potential biases inherent in the survey design. The method and the formula used to calculate the sample size should be fully documented	Yes	Yes	NA
	Implementation of the sampling activity	The sampling methods used should be fully documented including the related assumptions and uncertainties, and a justification for choosing the approach should be provided. Timeframe of the surveillance data and geographical clustering of the infection must to be reported. The sample collection period must comprise the whole year and the spatial distribution of the sampling must be homogeneous	Yes	Yes	NA
Methodology	Design prevalence (DP)	DP is specified in Annex II to Regulation (EU) No 1152/2011 and must be 1% or lower	Yes	Yes	NA
	Geographical epidemiologic unit	The geographic epidemiological unit(s) identified as target for the surveillance activity has to be clearly indicated and supported by justification	Yes	Yes	The whole territory of GB and NI were correctly considered each as one epidemiological unit in their respective analysis
	Methodology for calculation of area sensitivity	For the calculation of the area sensitivity, the diagnostic sensitivity should be set conservatively to the lowest value, excluding the lowest 20th percentile, from the ones reported in the scientific literature and related to the diagnostic tests implemented by the countries listed in Annex I of the Commission Delegated Regulation (EU) No 1152/2011. In this case, is 78% (EFSA AHAW Panel, 2015)	Yes	Yes	NA