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MSD of GFP-labeled MmpL5 using TIRF microscopy. Calculation of the MSD of MmpL5-GFP in the presence (blue line and bar) or absence (red line and bar) of MmpS5 (n = 25). (A) MSD-Δt plots of their foci. The closed symbols with error bars show the standard deviations of MSDs with all fixed (circles) or mobile (squares) foci. (Inset) The small graph presents the MSD-Δt plots of fixed MmpL5-GFP foci using a different y axis scale. (B) Distribution of the MSD of MmpL5-GFP foci at 330 ms.
