FIG 3.

Estimation of the number of GFP-labeled MmpL5 molecules in a single focus. (A) Stepwise photobleaching of single molecules in MmpL5-GFP foci in the presence of MmpS5. Gray dots show the intensity-Δt plots of MmpL5-GFP. Each step was identified using the edge detection filtered intensity (black line). The black arrows indicate the positions of predicted bleaching steps, and numbers indicate the intensity of the detected steps. Intensity is shown in arbitrary units. (B) Distribution plots of the fluorescent intensity of MmpL5-GFP at the first frame in the presence (blue bar) or absence (red bar) of MmpS5 (n = 58).